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Purines, Pyrimidines and Xanthines

Standard mixture solution for chromatography and mass spectrometry

Tags: uric acid metabolism standard mix, purines, pyrimidines and xanthine for calibration


Use: Liquid Chromatography (LC, HPLC, UHPLC) 

Use: Compounds identification, MS2 transitions,  retention time acquisition, in-house digital library, method development, quantification


Suitable for:  LCMS, UHPLC, LC-MS/MS, LC-QTOF 

Suitable for: Single quadrupole MS, triple quadrupole MS (QQQ), QTOF, Orbitrap

Suitable: Human metabolomics, plant metabolomics, microbiome analysis, single cell metabolomics, foodomics, food analysis, DNA fragment analysis, oligonucleotide sequencing, biomarker discovery, binding assays



HPLC-MS (single qaud):
-Find the retention time of molecules after changing the method
-Add the compounds to your in-house library for enhanced compound search/identification with RT and M/z value

-Use the standards to make calibration curves for quantification

HPLC-MS/MS (triple qaud):
-Acquire and optimize the transition ions for each molecule using your instrument specific settings
-Use the standards to make calibration curves for quantification


HPLC - High resolution (QTOF, Orbitrap):
-Use the standards to make calibration curves for quantification
-Add the compounds to your in-house library for enhanced search with RT and M/z value

Our Uric acid metabolism standard mixture contains molecules that are mapped in uric acid pathway including purines like adenosine, xanthosin and guanosin as well as other pyrimidine bases and their metabolites. The kit is designed to be used by common LCMS methods, but we recommend using MS/MS or high resolution techniques for best results, as some molecules show overlapped peaks because of isotopic pattern interference. We provide a HILIC method for this kit, although with an MS/MS or a high resolution MS, a C18 or a C8 column can be used. 


A kit comes in 3 mixture solutions. Each mix comes in 5 microampule of 0.2 mL quantity (total of 1.0 mL).

We provide methods of identification on HILIC in our interactive online Certificate of Analysis. The list of compounds with annotations and exact masses can be downloaded as a .csv file for fast transfer of information to your software. The top transition ions are also provided for all the molecules on the online specification page. We recommend the user to run the first set of the mixture to optimize thier and find their instrument specific transitions.


All of the CoA, SDS and methods come in digital format at our specification page which is product specific. No printed material is provided with this kit. The hard copy SDS is available upon request.  


You will need 9-425 vials (common autosampler vials) and caps to be able to run the samples directly. If your autosampler does not accept this type of vials, you will need to pipette out the solution to the compatible vessel. 


- 46 molecules in high purity, single peak
- Fully quantitative, 20 - 200 µg/mL in DMF solvent
5  High recovery sets with 15 molecules in each mix: Each set contains five microampules of 200 uL. Using microampules allows you to keep the unused amount in the sealed glass container. Each set contains 5 microampules, totalling to 15 microampules.
- Packed under argon
- Contains an internal standard to adjust your retention time with IS lock and system variation.
- No isobaric interference means there are no two compounds with the same molecular formula in a microampule. This design allows identification by low resolution single quad mass spectrometer without a need for digital libraries for identification.

Purines, Pyrimidines and Xanthines

  • List of compounds

    1. Cytosine  (HMDB0000630, CAS 71-30-7) (0.10mg/mL)
    2. Uracil  (HMDB0000300, CAS 66-22-8) (0.20mg/mL)
    3. 5-Methylcytosine  (HMDB0002894, CAS 554-01-8) (0.20mg/mL)
    4. 5-Aminoimidazole-4-carboxamide  (HMDB0003192, CAS 360-97-4) (0.10mg/mL)
    5. 3-Ureidopropionic acid  (HMDB0000026, CAS 462-88-4) (0.20mg/mL)
    6. Adenine hydrochloride  (HMDB0000034, CAS 2922-28-3) (0.10mg/mL)
    7. Hypoxanthine  (HMDB0000157, CAS 68-94-0) (0.10mg/mL)
    8. 5-(Hydroxymethyl)uracil  (HMDB0000469, CAS 4433-40-3) (0.10mg/mL)
    9. Guanine  (HMDB0000132, CAS 73-40-5) (0.02mg/mL)
    10. Oxipurinol  (HMDB0000786, CAS 2465-59-0) (0.10mg/mL)
    11. L-Hydroorotic acid  (HMDB0000528, CAS 5988-19-2) (0.10mg/mL)
    12. 3-Methylxanthine  (HMDB0001886, CAS 1076-22-8) (0.10mg/mL)
    13. Uric acid  (HMDB0000289, CAS 69-93-2) (0.02mg/mL)
    14. Theobromine  (HMDB0002825, CAS 83-67-0) (0.10mg/mL)
    15. Dihydrouracil  (HMDB0000076, CAS 504-07-4) (0.20mg/mL)
    16. Thymine  (HMDB0000262, CAS 65-71-4) (0.20mg/mL)
    17. 1,3-Dimethyluracil  (HMDB0002144, CAS 874-14-6) (0.10mg/mL)
    18. Orotic acid anhydrous  (HMDB0000226, CAS 65-86-1) (0.10mg/mL)
    19. Allantoin  (HMDB0000462, CAS 97-59-6) (0.10mg/mL)
    20. Theophylline  (HMDB0001889, CAS 58-55-9) (0.10mg/mL)
    21. Cytidine  (HMDB0000089, CAS 65-46-3) (0.10mg/mL)
    22. Uridine  (HMDB0000296, CAS 58-96-8) (0.10mg/mL)
    23. Caffeine  (HMDB0001847, CAS 21399) (0.10mg/mL)
    24. Adenosine  (HMDB0000050, CAS 58-61-7) (0.10mg/mL)
    25. Inosine  (HMDB0000195, CAS 58-63-9) (0.10mg/mL)
    26. Guanosine  (HMDB0000133, CAS 118-00-3) (0.10mg/mL)
    27. Xanthosine dihydrate  (HMDB0000299, CAS 146-80-5) (0.10mg/mL)
    28. Theacrine  (HMDB0004328, CAS 2309-49-1) (0.10mg/mL)
    29. 1-Methyl Adenosine  (HMDB0003331, CAS 15763-06-1) (0.10mg/mL)
    30. Purine  (HMDB0001366, CAS 120-73-0) (0.10mg/mL)

  • List of compounds (continued)

    31. Allopurinol  (HMDB0014581, CAS 315-30-0) (0.05mg/mL)
    32. 5-Formyluracil  (PUBCHEMCID304590, CAS 1195-08-0) (0.10mg/mL)
    33. N6-Methyladenine  (HMDB0002099, CAS 443-72-1) (0.05mg/mL)
    34. Xanthine  (HMDB0000292, CAS 69-89-6) (0.05mg/mL)
    35. N-Carbamoyl-DL-aspartic acid  (HMDB0000828, CAS 923-37-5) (0.10mg/mL)
    36. 5-Methyluridine  (HMDB0000884, CAS 1463-10-1) (0.10mg/mL)
    37. 2'-Deoxycytidine  (HMDB0000014, CAS 951-77-9) (0.10mg/mL)
    38. 2'-Deoxyuridine  (HMDB0000012, CAS 951-78-0) (0.10mg/mL)
    39. Thymidine  (HMDB0000273, CAS 50-89-5) (0.10mg/mL)
    40. 2'-Deoxyadenosine  (HMDB0000101, CAS 958-09-8) (0.10mg/mL)
    41. 2'-Deoxyinosine  (HMDB0000071, CAS 890-38-0) (0.10mg/mL)
    42. 2′-Deoxyguanosine  (HMDB0000085, CAS 961-07-9) (0.10mg/mL)
    43. 7-Methylguanosine  (HMDB0001107, CAS 20244-86-4) (0.05mg/mL)
    44. 3-Methyladenine  (HMDB0011600, CAS 5142-23-4) (0.10mg/mL)
    45. 5-Hydroxymethylcytosine  (PUBCHEMCID70751, CAS 1123-95-1) (0.10mg/mL)
    46. 1,3-Dimethyluric acid  (HMDB0001857, CAS 944-73-0) (0.10mg/mL)

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