Polyphenols: Flavonoids Standard Mixtures

Standard mixture solution for chromatography and mass spectrometry

Tags: polyphenols standard mixt, phenolic acids standard mix, phenolic alcohols standard mix

 

Use: Liquid Chromatography (LC, HPLC, UHPLC) 

Use: Compounds identification, MS2 transitions,  retention time acquisition, in-house digital library, method development

 

Suitable for:  LCMS, UHPLC, LCMS, LC-MS/MS, LC-QTOF 

Suitable for: Single quadrupole MS, triple quadrupole MS (QQQ), QTOF, Orbitrap

Suitable: Human metabolomics, plant metabolomics, foodomics, food analysis, volatilomics, cannabis and vape analysis, wine and beer analysis

 

Applications: 

 

HPLC-ELSD, HPLC-CAD
-Find the retention time of the molecules after changing the method
-Find the retention time of molecules after changing the column
-Use the standards to make calibration curves for quantification

 

HPLC-MS (single qaud):
-Find the retention time of molecules after changing the method
-Add the compounds to your in-house library for enhanced compound search/identification with RT and M/z value

 

HPLC-MS/MS (triple qaud):
-Acquire and optimize the transition ions for each molecule using your instrument specific settings
-Use the standards to make calibration curves for quantification

 

HPLC-MS/MS (QTOF):
-Use the standards to make calibration curves for quantification
-Add the compounds to your in-house library for enhanced search with RT and M/z value

 

 

 

The presence of polyphenolic micronutrients in food is shown to be beneficial to humans health due to their powerful anti-oxidation properties. MetaSci’s Flavonoids Mix contains 40 flavonoids including flavonols, flavonones and chalcones that are commonly found in plants, foods and health supplements. The standard mixtures come as 0.2mM - 1.0 mM methanolic solution and are suitable to use with HPLC methods utilizing a standard C18 column and MS, UV or ELSD detector. Each mixture is designed to have 10 compounds highly resolved on a C18 column with no isobaric interference which makes it to work even on a low resolution MS. The standard mixtures can be used for peak identification, making calibration curves for quantification and making an in-house digital library of spectra.


Why digital libraries (NIST, METLIN, etc.) are not enough?
Every instrument yields an analysis result specific to its brand, build, methods and other parameters. Digital libraries only contain spectra resulted from the instrument of its producer and lose quality as it is used for other machines. To produce the most accurate result for each instrument, a lab should run physical standards on every instrument and on each when the methods or conditions (column, solvent, pH, etc) is changed. 

 

 

  • 40 flavonoid and similars, high purity, single peak, completely resolved
  • Fully quantitative, 1mM in MeOH/DMF (2/1).
  • 4  High recovery sets with 10 flavonoid in each. Each set contains five microampules of 200 uL. Using microampules allows you to keep the unused amount in the sealed glass container. Each set contains 5 microampules, totalling to 20 microampules.
  • Packed under argon
  • Contains an internal standard to adjust your retention time with IS lock
  • No isobaric interference means there are no two compounds with the same molecular formula in a microampule. This design allows identification by low resolution single quad mass spectrometer without a need for digital libraries for identification.

Polyphenols: Flavonoids Standard Mixtures

  • 1. (+)-Catechin (Hydrate) (CAS 154-23-4) (1.0mM)
    2. Mangiferin (CAS 4773-96-0) (1.0mM)
    3. Naringin (CAS 10236-47-2) (1.0mM)
    4. Myricetin (CAS 529-44-2) (1.0mM)
    5. Baicalin (CAS 21967-41-9) (1.0mM)
    6. Quercetin (CAS 117-39-5) (1.0mM)
    7. Neohesperidin dihydrochalcone (CAS 20702-77-6) (1.0mM)
    8. Phloretin (CAS 60-82-2) (1.0mM)
    9. Kaempferol (CAS 520-18-3) (1.0mM)
    10. Baicalein (CAS 491-67-8) (1.0mM)
    11. (-)-Epigallocatechin gallate hydrate (CAS 989-51-5) (1.0mM)
    12. Polydatin (CAS 65914-17-2) (1.0mM)
    13. Hesperidin (CAS 520-26-3) (0.2mM)
    14. Diosmin (CAS 520-27-4) (0.2mM)
    15. Morin (CAS 480-16-0) (1.0mM)
    16. (+/-)-Naringenin (CAS 67604-48-2) (1.0mM)
    17. Luteolin (CAS 491-70-3) (1.0mM)
    18. Hesperetin (CAS 520-33-2) (1.0mM)
    19. Diosmetin (CAS 520-34-3) (1.0mM)
    20. Chrysin (CAS 480-40-0) (1.0mM)
    21. (-)-Epicatechin (CAS 490-46-0) (1.0mM)
    22. (+)-Taxifolin (CAS 480-18-2) (1.0mM)
    23. Phloridzin (CAS 60-81-1) (1.0mM)
    24. Fisetin (CAS 528-48-3) (1.0mM)
    25. Daidzein (CAS 486-66-8) (1.0mM)
    26. Resveratrol (CAS 501-36-0) (1.0mM)
    27. Apigenin (CAS 520-36-5) (1.0mM)
    28. 4'-Hydroxychalcone (CAS 2657-25-2) (1.0mM)
    29. Flavone (CAS 525-82-6) (1.0mM)
    30. 3-Hydroxyflavone (CAS 577-85-5) (1.0mM)
    31. Puerarin (CAS 3681-99-0) (1.0mM)
    32. Rutin (CAS 153-18-4) (0.2mM)
    33. Neohesperidin (CAS 13241-33-3) (1.0mM)
    34. Naringin dihydrochalcone (CAS 18916-17-1) (1.0mM)
    35. Equol (CAS 94105-90-5) (1.0mM)
    36. Genistein (CAS 446-72-0) (1.0mM)
    37. Isoliquiritigenin (CAS 961-29-5) (1.0mM)
    38. Formononetin (CAS 485-72-3) (1.0mM)
    39. Biochanin A (CAS 491-80-5) (1.0mM)
    40. trans-Chalcone (CAS 614-47-1) (1.0mM)

  • Additional information including methods, retention times, m/z transition ions, metabolite annotations, CoA, SDS, etc., are available at spec.metasci.ca or by scanning the QR code on your product.