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Amino Acids Standard Mixture

Standard mixture solution for chromatography and mass spectrometry

Tags: amino acids standard mix, amino acids standard mixture, amino acids standards for calibration


Use: Liquid Chromatography (LC, HPLC, UHPLC) 

Use: Compounds identification, MS2 transitions,  retention time acquisition, in-house digital library, method development, quantification


Suitable for:  LCMS, UHPLC, LCMS, LC-MS/MS, LC-QTOF 

Suitable for: Single quadrupole MS, triple quadrupole MS (QQQ), QTOF, Orbitrap

Suitable: Human metabolomics, plant metabolomics, microbiome analysis, single cell metabolomics, foodomics, food analysis, wine and beer analysis, plant based food analysis, biomarker discovery, binding assays




-Find the retention time of the molecules after changing the method
-Find the retention time of molecules after changing the column
-Use the standards to make calibration curves for quantification


HPLC-MS (single qaud):
-Find the retention time of molecules after changing the method
-Add the compounds to your in-house library for enhanced compound search/identification with RT and M/z value

-Use the standards to make calibration curves for quantification


HPLC-MS/MS (triple qaud):
-Acquire and optimize the transition ions for each molecule using your instrument specific settings
-Use the standards to make calibration curves for quantification


HPLC - High resolution (QTOF, Orbitrap):
-Use the standards to make calibration curves for quantification
-Add the compounds to your in-house library for enhanced search with RT and M/z value


Proteogenic and non-proteogenic alpha amino acids are commonly analyzed and quantified for various application. The MetaSci Amino Acids Standard Mixture Kit is designed to accelerate the identification and quantification by packing as many as compatible compounds in a mix, allowing multiple spectra recording in a single injection. The kit is composed of 60 amino acids, in three mixes. Each mix has 20 amino acids in it.  An internal standard, 

2-Ethoxyisonicotinic acid, is used in all mixes at the constant concentration of 50 ug/mL to normalize the variance in volume of injection. This internal standard is highly ionizable both at positive and negative mode, resulting in consistent response. 


The kit comes in two different types. The first type contains only the mixtures with each amino acid at 100ug/mL. The second type comes in a serially diluted series of mixtures starting at 100ug/mL followed by 50, 25, 10, 5, 2.5, 1.0, 0.5, 0.25 and 0.10 ug/mL solutions. The advantage of using the serial dilution is two folds: a) higher accuracy because the dilutions are made in controlled environment, with calibrated equipment and in higher volumes, resulting in lower inaccuracy in preparation;  b) all the dilutions are made with a buffer containing 50ug/mL of IS. The constant presence of IS at certain concentration from stock solutions to the last dilution makes it possible to accurately calculate any variance in the system and normalize the results. 

Another feature of this kit is the absence of any isobaric interference. Every amino acid molecule in each mix is at least 1 Da different from all other molecules which eliminates uncertainties in identification of molecules after changing method, column, solvent, pH, etc. For example, the well-known isobaric Leu and Ile are present in two different mixes, which removed the guesswork. Cystein and Homocystein are included in the kit and their accurate amount can be measured by calcuating the concentration of their dimers Cystine and Homocystine which is provided in calibrant series in a third ampule.  


A kit comes in 3 mixture solutions. Each mix comes in 5 microampule of 0.2 uL quantity (total of 1.0 mL). The calibrant series come in 5 microampules of 0.2 uL for each calibration point. There are a total of 10 calibration point (100ug/mL - 0.1ug/mL).


We provide multiple methods of identification on HILIC in our interactive online Certificate of Analysis. The list of compounds with annotations and exact masses can be downloaded as a .csv file for fast transfer of information to your software. The top two transitions are also provided for all the amino acids in the CoA.


All of the CoA, SDS and methods come in digital format at our specification page which is product specific. No printed material is provided with this kit. The hard copy SDS is available upon request.  


You will need 9-425 vials (common autosampler vials)  and caps to be able to run the samples directly. If your autosampler does not accept this type of vials, you will need to pipette out the solution to the compatible vessel. If you use plate based auto sampler, we can provide a custom 96 well plate that fits the micro ampoule for direct injection. 



  • 60 proteogenic and non-proteogenic amino acids, high purity, single peak, completely resolved
  • Fully quantitative, 100 ug/mL in ammonium chloride buffer.
  • 5  High recovery sets with 20 amino acids in each mix: Each set contains five microampules of 200 uL. Using microampules allows you to keep the unused amount in the sealed glass container. Each set contains 5 microampules, totalling to 25 microampules.
  • Packed under argon
  • Contains an internal standard to adjust your retention time with IS lock and system variation.
  • No isobaric interference means there are no two compounds with the same molecular formula in a microampule. This design allows identification by low resolution single quad mass spectrometer without a need for digital libraries for identification.

Amino Acids Standard Mixture

  • List of standard compounds

    1. Glycinamide (HMDB0062472, CAS 1668-10-6)
    2. Sarcosin (HMDB0000271, CAS 107-97-1)
    3. N,N-Dimethylglycine (HMDB0000092, CAS 1118-68-9)
    4. DL-Homocysteine thiolactone (HMDB0002287, CAS 6038-19-3)
    5. L-Homocysteine (HMDB0000719, CAS 6027-13-0)
    6. trans-4-L-Hydroxyproline (Hydroxyproline, HMDB0000725, CAS 51-35-4)
    7. DL-Homocysteine (HMDB0000742, CAS 454-29-5)
    8. N,N-dimethyl-L-Valine (PUBCHEMCID11008044, CAS 2812-32-0)
    9. L-Isoglutamine (PUBCHEMCID445883, CAS 636-65-7)
    10. L-Cysteic acid (HMDB0002757, CAS 23537-25-9)
    11. L-Citrulline (HMDB0000904, CAS 372-75-8)
    12. N-Formyl-L-methionine (HMDB0001015, CAS 4289-98-9)
    13. Phosphoserine (HMDB0000272, CAS 407-41-0)
    14. L-Homoarginine (HMDB0000670, CAS 1483-01-8)
    15. L-Homocitrulline (HMDB0000679, CAS 1190-49-4)
    16. N-Carbamyl-L-Glutamic Acid (HMDB0062795, CAS 1188-38-1)
    17. Tryptophanamide (H-Trp-NH2.HCl, HMDB0013318, CAS 5022-65-1)
    18. 3-Chloro-L-Tyrosine (HMDB0001885, CAS 7423-93-0)
    19. 3-Nitro-L-tyrosine (Nitrotyrosine, HMDB0001904, CAS 621-44-3)
    20. O-Phospho-L-tyrosine (Phosphotyrosine, HMDB0006049, CAS 21820-51-9)
    21. Glycine (HMDB0000123, CAS 56-40-6)
    22. L-Alanine (HMDB0000161, CAS 56-41-7)
    23. 2-Aminoisobutyric acid (HMDB0001906, CAS 62-57-7)
    24. L-Serine (HMDB0000187, CAS 56-45-1)
    25. L-Proline (HMDB0000162, CAS 147-85-3)
    26. L-Valine (HMDB0000883, CAS 72-18-4)
    27. L-Threonine (HMDB0000167, CAS 72-19-5)
    28. L-Cysteine (HMDB0000574, CAS 52-90-4)
    29. L-Leucine (HMDB0000687, CAS 61-90-5)
    30. L-Asparagine (HMDB0000168, CAS 70-47-3)
    31. L-Aspartic acid (HMDB0000191, CAS 56-84-8)
    32. S-Methyl-L-cysteine (HMDB0302211, CAS 1187-84-4)
    33. L-Lysine (HMDB0000182, CAS 56-87-1)
    34. L-Glutamic acid (HMDB0000148, CAS 56-86-0)
    35. L-Methionine (HMDB0000696, CAS 63-68-3)
    36. L-Histidine (HMDB0000177, CAS 71-00-1)
    37. L-Phenylalanine (HMDB0000159, CAS 63-91-2)
    38. L-Arginine (HMDB0000517, CAS 74-79-3)
    39. L-Tyrosine (HMDB0000158, CAS 60-18-4)
    40. L-Tryptophan (HMDB0000929, CAS 73-22-3)
    41. β-Alanine (HMDB0000056, CAS 107-95-9)
    42. 1-Aminocyclopropanecarboxylic acid (HMDB0036458, CAS 22059-21-8)
    43. L-α-aminobutyric acid (Homoalanine, HMDB0000452, CAS 1492-24-6)
    44. L-Norvaline (HMDB0013716, CAS 6600-40-4)
    45. Taurine (HMDB0000251, CAS 107-35-7)
    46. L-Isoleucine (HMDB0000172, CAS 73-32-5)
    47. L-Ornithine (HMDB0000214, CAS 3184-13-2)
    48. L-Glutamine (HMDB0000641, CAS 56-85-9)
    49. N-Methyl-D-aspartic acid (HMDB0002393, CAS 6384-92-5)
    50. S-Methyl-L-cysteine sulfoxide (HMDB0029432, CAS 6853-87-8)
    51. L-Theanine  (HMDB0034365, CAS 3081-61-6)
    52. Ureidosuccinic acid (N-Carbamoyl-DL-aspartic acid, HMDB0000828, CAS 923-37-5)
    53. Carbocysteine (HMDB0029415, CAS 638-23-3)
    54. DL-m-Tyrosine (meta-Tyrosin, HMDB0059720, CAS 775-06-4)
    55. N-Epsilon-acetyl-l-lysine (HMDB0000206, CAS 692-04-6)
    56. Selenomethionine (HMDB0003966, CAS 3211-76-5)
    57. L-Dopa (HMDB0000181, CAS 59-92-7)
    58. L-5-Hydroxytryptophan (HMDB0000472, CAS 895096)
    59. L-Cystine (HMDB0000192, CAS 56-89-3)
    60. L-Homocysteine (HMDB0000575, CAS 6027-13-0)

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